TOOLSignal Boosting Buffer
TOOLSignal Boosting Buffer is the best alternative for conventional blocking buffer and it improves the performance of your Western blot experiments for all types of antibodies. Simply incubate this buffer with primary or secondary antibodies without using any blocking buffer (milk or BSA) to obtain the effective blocking result. TOOLSignal Boosting Buffer shortens the experimental time and reduces the use of antibodies without compromising Western Blot signal intensity.
產品編號
規格
TW-NB250
250 mL
產品編號
TW-NB250
規格
250 mL
產品特色
- No blocking step.
- No background.
- High sensitivity.
- Save time.
- Save antibody
實驗數據
Fig 1. Lane 1: RD cells. Lane 2: tual1- knockout RD cells.
Protein input: 30ug.
1° antibody: Anti-Stau1 antibody 1:1000, Anti-beta-actin antibody 1:10000
2° antibody: 1:5000
Fig 2. Cell line: BHK-21.
1° antibody: KDEL Receptor (N1C1) 1:1000
2° antibody: Goat anti Rabbit 1:10000
Protein input: lane 1,3= 30ug ; lane 2,4= 60ug
Fig 3. Cell line: BHK-21.TOOLSignal boosting buffer:
1° antibody 1:2000 in buffer, RT 1 hr
2° antibody 1:1000 in buffer, RT 1 hr
5% milk:
Blocking 30 min
1° antibody 1:2000 in PBST, RT 1 hr.
2° antibody 1:1000 in PBST, RT 1 hr.
Protein input: 30ug.
1° antibody: Anti-Stau1 antibody 1:1000, Anti-beta-actin antibody 1:10000
2° antibody: 1:5000
Fig 2. Cell line: BHK-21.
1° antibody: KDEL Receptor (N1C1) 1:1000
2° antibody: Goat anti Rabbit 1:10000
Protein input: lane 1,3= 30ug ; lane 2,4= 60ug
Fig 3. Cell line: BHK-21.TOOLSignal boosting buffer:
1° antibody 1:2000 in buffer, RT 1 hr
2° antibody 1:1000 in buffer, RT 1 hr
5% milk:
Blocking 30 min
1° antibody 1:2000 in PBST, RT 1 hr.
2° antibody 1:1000 in PBST, RT 1 hr.