EasyPrep plant and fungi DNA kit adopts a unique buffer system and is especially suitable for the purification of DNA from lyophilized or fresh plant tissues. The kit avoids the use of hazardous organic solvents such as phenol and removes the vast majority of proteins and other organic purities. There is no limit to starting amount of samples; the experimenter could adjust that according to actual demand. DNA purified with this kit is ready to use in downstream applications including enzyme digestion reaction, PCR, library construction, southern blot, and so on.