TOOLS 5X EvaGreen qPCR Mix with ROX
TOOLS 5X EvaGreen qPCR Mix with ROX is optimized for real-time quantitative PCR assays. The ready-to-load mix includes TOOLS HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye, and ROX dye according to system requirements. Only water, template, and primers need to be added. TOOLS HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
產品編號
規格
TF-5EVR250
1 mL
產品編號
TF-5EVR250
規格
1 mL
產品特色
- Fast qPCR results and high performance.
- Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility.
- Robust discrimination and reproducibility.
- Unique "release-on-demand" DNA binding mechanism of EvaGreen dye enables superior PCR and melt curve analysis results.
- Novel chemically-modified hot start Taq, requiring only 15 minutes to activate.
- Compatible with both fast and regular cycling protocols.
- EvaGreen dye in the master mix can also serve as a gel stain - Analyze your PCR product by gel electrophoresis without the need to add another gel stain.