TOOLS Ultra High Fidelity PCR Master Mix is is a new generation superior enzyme based on Ultra Hi-Fi DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to TOOLS Ultra High Fidelity PCR Master Mix Ultra Hi-Fi greatly improve its long-fragment amplification ability, specificity, and PCR yield. TOOLS Ultra High Fidelity PCR Master Mix Ultra Hi-Fi is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. . The amplification error rate of TOOLS Ultra High Fidelity Super-Fidelity DNA PolymeraseP Ultra Hi-Fi is 53-fold lower than that of conventional Taq and 6-fold lower than that of nPfu. In addition, TOOLS Ultra High Fidelity Super-Fidelity DNA Polymerase Ultra Hi-Fi has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. TOOLS Ultra High Fidelity DNA Polymerase P Ultra Hi-Fi contains two monoclonal antibodies inhibiting the 5 ́→3 ́ polymerase activity and 3 ́→5 ́ exonuclease activity at room temperature, which enable TOOLS Ultra High Fidelity DNA PolymeraseUltra Hi-Fi to perform greatly-specific Hot-Start PCRs. TOOLS Ultra High Fidelity PCR MasterMix contains Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in this product are capable of repeated freeze-thaw cycles.