TOOLS Ultra High Fidelity DNA Polymerase is a new generation superior enzyme based on Ultra High Fidelity DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Ultra High Fidelity DNA Polymerase greatly improve its long-fragment amplification ability, specificity, and PCR yield. Ultra High Fidelity DNA Polymerase is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Ultra High Fidelity DNA Polymerase is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Ultra High Fidelity DNA Polymerase has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Ultra High Fidelity DNA Polymerase contains two monoclonal antibodies inhibiting the 5 ́→3 ́ polymerase activity and 3 ́→5 ́ exonuclease activity at room temperature, which enable Ultra High Fidelity DNA Polymerase to perform greatly-specific Hot-Start PCRs. The amplification generates blunt-ended products, which are compatible with TOOLS UltraFast PCR cloning kit (TTC-CA15).