客製兔單株抗體(Rabbit Monoclonal Antibody Production)
ABclonal客製化兔單株抗體，透過單B細胞的第四代兔單克隆抗體篩選技術平臺SMab™ (Single cell-based monoclonal antibody)用於研發與診斷試劑開發，與傳統融合瘤技術比較，可以高效生產具有優越特異性、靈敏度高和應用性廣的重組抗體。SMab兔單株抗體四大優勢：1)高通量篩選可以快速分選和培養多達10000個單B細胞、2)經過設計的免疫流程和抗原富集過程 ， 能夠大大提高獲得ELISA陽性clone的比例(可高達40%)、3)利用專利技術開發的B細胞培養上清 ，進行細胞的大量培養與複製、4)快速獲得抗體的序列，能夠在早期進行初步的人源化改造。
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服務特色
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免費抗原設計：根據您的實驗設計與目的，並由ABclonal專業團隊提供完整的抗原分析與預測報告，在製備抗體前設計出最適合的抗原。
- 兔單株抗體優勢：高親和性(High affinity)、優越的特異性(Superior specificity)、廣泛的抗原表面位(Broad epitope recognition)
- 保證結果 ：當抗體以 1:1000 稀釋時，可檢測到 1 ng 蛋白質抗原或 100 ng 肽抗原。
- 抗體應用性廣：抗體可應用在WB, ELISA, IHC, IF, ICC, IP, ChIP；臨床診斷試劑開發、抗體藥開發
服務流程
抗體製備流程
Note
服務交期：18-26 weeks
服務交期：18-26 weeks
服務要求
- 提供預合成的抗原，請填寫「抗原資訊表」
- 若需要自行提供抗原請填寫「Material Submission Form」
產品交付
- 抗原：0.1 mg蛋白抗原或2mg胜肽抗原
- 血清：100 μl 免疫前/後血清
- 抗體：100 μg/抗體，1-3支 (protein A/G purified)
- 載體：15 μl/載體，1-3支 (VH & VL Vector)
- 抗體序列：1-3支抗體序列報告
- 報告：抗體驗證報告書一份
實際案例分享
Case Study1-Rabbit mAbs Against CCL18
Most rabbit monoclonal antibodies available in the current market face challenges such as having low affinity and exhibiting clear cross-reactivity. However, our client entrusted us to develop a high-affinity, specific rabbit monoclonal antibodies against CCL18 – a protein that is highly homologous with other members in the CC family. By using methods such as antigen-binding ELISA assay and using non-homologous peptides as boosts, the following results were achieved.
Case Study2-Efficient Screening Of Anti-Hapten Rabbit mAbs
Rabbits are better than mice in presenting nonpeptic haptens because of their expression of proteins in the CD1 family. For this project, results achieved include:
Case Study3-Quick Discovery Of PD-L1-Blocking Antibodies
PD1 is a popular target in life science research that involves in the ligand-receptor interaction. For this project, results achieved include:
Most rabbit monoclonal antibodies available in the current market face challenges such as having low affinity and exhibiting clear cross-reactivity. However, our client entrusted us to develop a high-affinity, specific rabbit monoclonal antibodies against CCL18 – a protein that is highly homologous with other members in the CC family. By using methods such as antigen-binding ELISA assay and using non-homologous peptides as boosts, the following results were achieved.
- 1200 single B cell clones
- 119 positive clones (10%)
- 26 selected clones
- 24 pairs of mAb genes
- 22 functional mAbs
- 17 specific anti-CCL18 mAbs
- 6 multi-assay mAbs
Case Study2-Efficient Screening Of Anti-Hapten Rabbit mAbs
- 1334 single B cell clones
- 100 positive clones (7.4%)
- 18 selected clones
- 17 pairs of mAb genes
- 16 functional mAbs
Case Study3-Quick Discovery Of PD-L1-Blocking Antibodies
PD1 is a popular target in life science research that involves in the ligand-receptor interaction. For this project, results achieved include:
- 2304 single B cell clones
- 386 positive clones (7.4%)
- 19 Strong PDL-1 blocking clones (4.9%)
- 29 Medium PDL-1 blocking clones (7.5%)
