TOOLSharp RNA Extractor
TOOLSharp RNA Extractor is the most efficient reagent for isolation of total RNA from human, animal, plant, bacterial and viral origin samples. This unique reagent provides a higher yield and quality of isolated RNA than traditional reagents based on the single-step method. TOOLSharp RNA Extractor isolates pure and undegraded RNA that is ready for the following application without DNase treatment. TOOLSharp RNA Extractor separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. A biological sample is homogenized or lysed in TOOLSharp RNA Extractor. DNA, proteins, polysaccharides, and other molecules precipitate out of the homogenate/ lysate by addition of water and removing it by centrifugation. The pure RNA is isolated from the resulting supernatant by alcohol precipitation, followed by elution.
產品特色
- Fast: Obtain RNA in 1 hour
- No chloroform: To remove DNA, protein, polysaccharides, and other contaminants only with water.
- Total RNA (including mRNA, rRNA, microRNA, etc.) is isolated in one tube.
- High RNA yield and purity.
- Suitable for any sample type.
- Low sample amount limitation.
- Multiple applications: RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.