EasyPrep EndoFree Maxi Plasmid Extraction kit is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt and uses Buffer PB4 and Filtration Column CS to wipe off the endotoxin and protein impurity effectively. This product is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells. 
Recommended bacterial culture volume: 500~1000ug plasmid with 100ml bacterial culture for high-copy vectors; and 200~600ug plasmid with 200ml bacterial culture for low-copy vectors.