TOOLS Quick RT Kit
TOOLS Quick RT Kit is designed for fast first-strand cDNA synthesis with high efficiency and is able to rapidly remove genomic DNA (gDNA). The kit is designed for 2-step RT-qPCR. The 5× Mix contains Buffer, dNTPs, TOOLScript Reverse Transcriptase, and RNase inhibitor. Random primers, Oligo-dT primers. It takes only 15 min (50°C) to complete the synthesis of first-strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables the efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.
產品特色
- Fast: Complete cDNA synthesis in 15 minutes
- Easy: Only contains 2 bottles
- High efficiency: Can work on GC-rich and secondary structure templates
- Flexible: Can use temperature from 50-55°C
實驗數據
產品原理
- One-bottle Solution: no premixing of components is required.
- Lower Toxicity: cells can be use for others experiments after CCK-8 assay.
- Hight Sensitivity: CCK-8 is the highest sensitive dye for cell-based assay.
- Many Applications: Can be used on cell proliferation, cell toxicity, drug selection experiments.
- Data Repeatability: results of the data has been confirmed many times and can be repeated....