TOOLS 2× Ultra Taq PCR MasterMix contains Ultra-Taq DNA Polymerase, dNTP, and an optimized buffer system containing 3’→5’exonuclease activity. Its fidelity is 6 times greater than that of Taq DNA Polymerase. Compared with Taq DNA Polymerase, Ultra-Taq DNA DNA polymerase has stronger amplification performance, higher sensitivity, and is more tolerant of impurities within 5 kb amplifying range. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. It can amplify up to 10 kb from human genomic DNA or up to 15 kb from λ DNA. Protective agents in the TOOLS 2× Ultra Taq PCR MasterMix enable the resistance to repeated freeze-thaw cycles