TOOLSFect transfection reagent
TOOLSFect Transfection Reagent is a powerful material that delivers DNA into a wide variety of cell lines with high efficiency and low toxicity. The additional steps, such as removal of serum or culture medium and washing or changing of the medium after introducing the reagent/DNA complex, are not required when using TOOLSFect Transfection Reagent.
產品編號
規格
TTM-TF01
1 mL
產品編號
TTM-TF01
規格
1 mL
產品特色
1. 方便：轉染前、後皆無須更換細胞培養基
2. 輕鬆：產品配有獨特配方working solution，無須額外準備其他無血清培養基作為溶液
3. 穩定：實驗證明，轉染後的細胞存活率高
4. 高效：經多種細胞株證實，成功轉染率高
2. 輕鬆：產品配有獨特配方working solution，無須額外準備其他無血清培養基作為溶液
3. 穩定：實驗證明，轉染後的細胞存活率高
4. 高效：經多種細胞株證實，成功轉染率高
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實驗數據
High Transfection efficiency
Figure 1. Transfection of GFP-expressing plasmid into (A) H4, (B) U87, (C) U118MG, (D) GL-261, (E) 9L/lacZ, (F) RG2 brain tumor cell line with TOOLSFect and leading transfection reagent, respectively. (G) The Bar graphs show the percentage of cells expressing GFP after transfection with a GFP-expressing plasmid. TOOLSFect transfection reagent demonstrates significantly higher transfection performance compared to leading transfection reagent in various cells lines derived from brain tumors. Over 80% of the panel is easy to transfect with TOOLSFect transfection reagent. The transfection is performed in a 12-well plate format (1×105 cells/well) using 3 µg plasmid/well and 3 µl transfection reagent respectively following the individual protocols.
Figure 2. U87MG spheres were transfected using TOOLSFect transfection reagent. Cells were visualized by fluorescence microscopy and TOOLSFect transfection reagent demonstrates significantly high transfection performance in the sphere.
Figure 3. Transfection of GFP-expressing plasmid into (Left to right) CH0-K1, HT1080, COS-7 cell line with TOOLSFect. The Transfection efficiency is determined by flow cytometry detecting GFP after transfection. Over 80% of the panel is easy to transfect with TOOLSFect transfection reagent. The transfection is performed in a 12-well plate format (1×10^5 cells/well) using 3 µg plasmid/well and 3 µl transfection reagent respectively following the individual protocols.
Transfect HUVEC
TOOLSFect transfection reagent and Competitor agents were used on Human umbilical vein endothelial cells (HUVEC) with a GFP-expressing plasmid in a 12-well plate format (1.2×10^5 cells/well), using 3 µg plasmid/well and the recommended protocols for each reagent. (A) GFP expression was analyzed 48 hours post-transfection, and (B) the Transfection efficiency is determined by flow cytometry detecting GFP after transfection. (C) Microphotograph represents cell morphology and viability in HUVEC post-transfection. (D) The Bar graphs representing the percentage of cells expressing GFP and viability after transfection shows TOOLSfect performs great transfection and low cytotoxicity.
Application in Lentivirus production
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TOOLSFect transfection reagent and Competitor agents were used to transfect plasmid for lentivirus production, pCMV-ΔR8.91 (12 kb), pMD.G (6.5 kb), and pLAS2w.RFP-C.Pneo (9 kb) into 293T cells in a 6-well plate format. The recommended protocols for each reagent were applied. The assembled lentivirus of RFP was collected and further used to infect the cells and (A) (B) RFP expression was analyzed 72 hours after lentivirus infection with different magnifications. (Table) The amounts of materials are recommended for lentivirus production with TOOLSFect.
